Composite
Part:BBa_M36553
Designed by: Aprotim Cory Bhowmik, Cali Nguyen, Zoe Lee-Chiong Group: Stanford BIOE44 - S11 (2015-10-22)
OYE6 Composite Sequence
Consists of multiple parts to produce the Old Yellow Enzyme 6 (OYE6) to reduce alpha-beta unsaturated C=C double bonds. Specifically consists of a rhamnose-inducible promoter (P_rha_BAD), a strong RBS, the OYE6 coding sequence, a His_* tag, and a termination sequence from T7.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 91
Illegal EcoRI site found at 1199 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 91
Illegal EcoRI site found at 1199 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 91
Illegal EcoRI site found at 1199 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 91
Illegal EcoRI site found at 1199 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 91
Illegal EcoRI site found at 1199
Illegal NgoMIV site found at 314
Illegal NgoMIV site found at 547
Illegal NgoMIV site found at 1165
Illegal AgeI site found at 140 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |